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Detection of circulating tissue factor and factor VII in a normal population
S Albrecht1, M Kotzsch, G Siegert
1Institut für Pathologie, Technische Universität Dresden, Germany.
Thrombosis and Haemostasis
|May 1, 1996
Summary
This study found no correlation between plasma tissue factor (TF) and factor VII (FVII) levels in healthy adults. While factor VII activity increased with age, TF concentration did not show age or gender differences.
Area of Science:
- Hematology
- Biochemistry
- Immunology
Background:
- Plasma tissue factor (TF) and factor VII (FVII) are crucial in hemostasis.
- Understanding their relationship and age-related changes is important for cardiovascular health.
- Previous studies have suggested potential links, but comprehensive analysis in healthy populations is needed.
Purpose of the Study:
- To investigate the correlation between plasma tissue factor (TF) concentration and factor VII concentration (FVIIag) and activity (FVIIc) in healthy adults.
- To assess the influence of age and gender on these hemostatic factors.
- To characterize immunoassays for TF and FVIIag determination.
Main Methods:
- Developed and characterized immunoassays using monoclonal antibodies (mAbs) for TF and FVIIag.
- Measured TF concentration, FVIIag, and FVIIc (clotting test) in 498 healthy volunteers (age 17-64).
- Analyzed data for age- and gender-related differences and correlations between TF and FVII parameters.
Main Results:
- Mean TF concentration was 172 ± 135 pg/ml, with no significant age or gender differences.
- Mean FVIIc was 110 ± 15% and FVIIag was 0.77 ± 0.19 µg/ml.
- FVII activity increased with age, but FVII concentration did not correlate with age. A strong correlation existed between FVIIc and FVIIag.
- The FVIIc/FVIIag ratio differed significantly between men and women but was not age-dependent.
- No significant correlation was found between plasma TF and FVII parameters.
Conclusions:
- Plasma TF concentration is independent of age and gender in healthy adults.
- Factor VII activity, but not concentration, increases with age in this population.
- Despite strong correlation between FVII activity and concentration, TF does not correlate with FVII levels, suggesting distinct regulatory mechanisms.