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[Viability and antigenic changes in the skin after preservation under different temperatures]
Zhonghua Zheng Xing Shao Shang Wai Ke Za Zhi = Zhonghua Zheng Xing Shao Shang Waikf [I.E. Waike] Zazhi = Chinese Journal of Plastic Surgery and Burns
|November 1, 1995
Summary
Cryopreservation of skin at -196°C significantly enhances viability compared to higher temperatures. Lower storage temperatures preserve skin antigenicity, crucial for transplantation and research.
Area of Science:
- Cryobiology
- Tissue Engineering
- Immunology
Background:
- Preserving biological tissues like skin is vital for transplantation and research.
- Understanding the impact of storage conditions on tissue viability and antigenicity is crucial.
Purpose of the Study:
- To compare the viability of human cadaver and guinea pig skin stored at various temperatures (4°C, -20°C, -80°C, -196°C).
- To assess the effect of different storage temperatures on skin antigenicity.
Main Methods:
- Oxygen consumption and succinate dehydrogenase content assays were used to measure skin viability.
- Microimmunoelectrophoresis and rocket immunoelectrophoresis were employed to evaluate antigenicity.
Main Results:
- Skin cryopreserved at -196°C exhibited significantly higher mean viability (70.8%) compared to storage at 4°C (61%) and other tested temperatures (P < 0.05 or 0.01).
- Antigenicity varied across storage groups, with fresh skin showing the highest antigenicity.
- A negative correlation was observed between storage temperature and skin antigenicity; higher temperatures resulted in lower antigenicity.
Conclusions:
- Cryopreservation at -196°C is superior for maintaining skin viability.
- Lower storage temperatures are essential for preserving skin antigenicity, which is compromised at higher temperatures.