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Related Experiment Videos

EGF-receptors in human normal and pathological thyroid tissue

K Westermark1, M Lundqvist, G Wallin

  • 1Department of Internal Medicine, University Hospital, Uppsala, Sweden.

Histopathology
|March 1, 1996
PubMed
Summary

Epidermal growth factor receptor (EGFR) expression was analyzed in various human thyroid tissues. While EGFR mRNA levels remained consistent, EGFR protein staining was stronger in neoplastic tissues, suggesting post-translational regulation in thyroid diseases.

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Area of Science:

  • Endocrinology
  • Oncology
  • Molecular Biology

Background:

  • The epidermal growth factor receptor (EGFR) plays a role in cell growth and differentiation.
  • Altered EGFR expression is implicated in various cancers, including thyroid malignancies.

Purpose of the Study:

  • To investigate EGFR expression and localization in normal and pathological human thyroid tissues.
  • To determine if EGFR expression levels correlate with different thyroid conditions, including goiter and carcinoma.
  • To explore the potential role of EGFR in thyroid neoplasia and goiter development.

Main Methods:

  • Immunohistochemistry was performed on cryosections of human thyroid tissues, including normal, nodular goiter, toxic goiter, adenoma, follicular carcinoma, papillary carcinoma, and poorly differentiated carcinoma.

Related Experiment Videos

  • Northern blot analysis was used to quantify EGFR mRNA levels in various thyroid tissues and anaplastic carcinoma cell lines.
  • EGFR immunoreactivity and localization were assessed in all examined tissues.

    • Main Results:

    • EGFR immunoreactivity was detected in all examined thyroid tissues.
    • No significant difference in EGFR mRNA levels was observed between normal and abnormal thyroid tissues.
    • EGFR protein staining intensity was weaker in normal thyroid tissue compared to adjacent neoplastic areas, indicating post-translational upregulation in tumors.
    • A strong EGFR staining was also noted in hyperfunctioning thyroid glands (toxic goiter).
    • EGFR localization differed: basal/basolateral in normal and toxic diffuse goiter, pericellular/cytoplasmic in neoplastic tissues, and varied in nodular goiter.

    Conclusions:

    • The study suggests that EGFR upregulation at the post-translational level occurs in thyroid neoplasms.
    • A non-polarized EGFR location in neoplastic tissues may indicate a loss of normal epithelial cell polarity and early dedifferentiation.
    • EGFR is proposed to play a role in the development of thyroid neoplasias and goiter formation.