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Assay for Adhesion and Agar Invasion in S. cerevisiae
Published on: November 8, 2006
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Saccharomyces cerevisiae TEC1 is required for pseudohyphal growth
V Gavrias1, A Andrianopoulos, C J Gimeno
1Myco Pharmaceuticals Inc., Cambridge, Massachusetts 02139, USA.
Molecular Microbiology
|March 1, 1996
Summary
The fungal developmental regulator AbaA from Aspergillus nidulans triggers pseudohyphal growth in yeast. This yeast
Area of Science:
- * Molecular biology
- * Mycology
- * Genetics
Background:
- * Eukaryotic organisms share homologous DNA-binding domains in developmental transcription factors.
- * The ATTS/TEA class of transcription factors plays a role in development.
- * Filamentous fungi like Aspergillus nidulans possess key developmental regulators.
Purpose of the Study:
- * To investigate the function of the Aspergillus nidulans developmental regulator AbaA in Saccharomyces cerevisiae.
- * To determine the role of the Saccharomyces cerevisiae homologue, TEC1p, in pseudohyphal development.
- * To elucidate the interaction between TEC1p and STE12p in yeast morphological transitions.
Main Methods:
- * Gene expression analysis of AbaA in Saccharomyces cerevisiae.
- * Functional assays for pseudohyphal development in yeast.
- * Genetic analysis involving TEC1p and STE12p.
Main Results:
- * AbaA from Aspergillus nidulans successfully induced pseudohyphal development in Saccharomyces cerevisiae.
- * The Saccharomyces cerevisiae homologue, TEC1p, was found to be essential for this induced morphological transition.
- * Evidence suggests TEC1p collaborates with STE12p to drive pseudohyphal development.
Conclusions:
- * The developmental regulator AbaA can functionally substitute for its yeast homologue in inducing pseudohyphal growth.
- * TEC1p is a key mediator of AbaA-induced pseudohyphal development in Saccharomyces cerevisiae.
- * The conserved function of ATTS/TEA transcription factors highlights their importance in eukaryotic development.
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