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Peripheral blood mononuclear cells develop into multinucleated osteoclasts in tissue culture
1Department of Neurobiology, University of California, Los Angeles, USA.
The Anatomical Record
|May 1, 1996
Summary
Peripheral blood mononuclear cells can form multinucleated osteoclasts in developing bone cultures. These specialized bone-resorbing cells require bone presence to develop in vitro.
Area of Science:
- Cell Biology
- Developmental Biology
- Bone Biology
Background:
- Osteoclasts, responsible for bone resorption, originate from mononuclear cells.
- Previous research indicates hemopoietic bone marrow and peripheral blood as sources for osteoclast precursors.
Purpose of the Study:
- To investigate the potential of peripheral blood mononuclear cells to form osteoclasts.
- To demonstrate osteoclastogenesis in vitro using fetal mouse calvaria explants.
Main Methods:
- Fetal mouse calvaria were cultured for 9 days to create an osteoclast-free bone environment.
- Peripheral blood mononuclear cells were isolated using Ficoll-Paque density gradient.
- Mononuclear cells were co-cultured with the prepared calvarial explants.
Main Results:
- Multinucleated cells with osteoclast characteristics were observed adjacent to woven bone after 10 days of co-culture.
- The experimental group showed an average of two osteoclasts per million mononuclear cells.
- No osteoclasts were detected in the control group lacking mononuclear cells.
Conclusions:
- Peripheral blood mononuclear cells possess the capacity to differentiate into multinucleated osteoclasts.
- Osteoclast development from these cells is dependent on the presence of bone tissue.