Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Formation of apatite-collagen complexes

Y Doi1, T Horiguchi, Y Moriwaki

  • 1Department of Dental Materials and Technology, School of Dentistry, Asahi University, Gifu, Japan.

Journal of Biomedical Materials Research
|May 1, 1996
PubMed
Summary

Scientists created a new type of bone substitute by combining collagen and apatite. They used a special solution to encourage apatite to form on collagen fibers. The resulting material was elastic and did not fall apart when tested with tools or in blood. When placed in muscle tissue, it dissolved safely without harming surrounding cells. The material could be useful for repairing bone in the mouth and jaw areas.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence for Isospin Violation and Measurement of CP Asymmetries in B→K^{*}(892)γ.

Physical review letters·2017
Same author

IL4Rα and ADAM33 as genetic markers in asthma exacerbations and type-2 inflammatory endotype.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2017
Same author

A Novel Rearranged Taxoid from Needles of the Japanese Yew, Taxus cuspidata Sieb. et Zucc.

Bioscience, biotechnology, and biochemistry·2016
Same author

Four Novel Taxane Diterpenoids from the Needles of Japanese Yew, Taxus cuspidata.

Bioscience, biotechnology, and biochemistry·2016
Same author

Isolation and characterization of dental epithelial cells derived from amelogenesis imperfecta rat.

Oral diseases·2015
Same author

Measurement of the Direct CP Asymmetry in B¯→X(s+dγ) Decays with a Lepton Tag.

Physical review letters·2015

Area of Science:

  • Biomaterials in regenerative medicine
  • Tissue engineering for bone repair
  • Biomineralization in orthopedic research

Background:

Current bone substitutes lack the structural and functional similarity to natural bone. Existing materials often fail to mimic the collagen-apatite composite found in native bone tissue. Scientists have long sought to develop synthetic alternatives that better replicate the mechanical and biological properties of bone. While natural bone contains collagen fibers coated with apatite crystals, most artificial substitutes lack this layered structure. Previous studies have explored various methods to induce apatite formation on collagen matrices. However, these approaches have not fully achieved the desired mechanical stability or biocompatibility. The challenge lies in creating a complex that remains intact under physical stress and degrades safely in vivo. This gap motivated researchers to develop a new method for synthesizing apatite-collagen composites that could serve as improved bone graft materials.

Purpose Of The Study:

The goal of this research was to create an apatite-collagen complex that mimics the composition of natural bone. The study aimed to test whether crosslinked collagen could support apatite deposition under controlled conditions. Researchers wanted to determine if the resulting complex could maintain structural integrity and resist disintegration in biological fluids. They also sought to assess the complex's resorption behavior and biocompatibility in a living system. The study focused on using calcium beta-glycerophosphate solutions at physiological pH and temperature. The objective was to evaluate the feasibility of this method for producing bone substitutes. The team aimed to compare the mechanical properties of the complex with those of native bone. Ultimately, the study aimed to provide a foundation for developing new materials for periodontal and alveolar bone repair.

Keywords:
bone substitute materialsapatite formationcollagen crosslinkingtissue engineering

Frequently Asked Questions

The complex gained approximately twice the weight of the collagen matrix from apatite deposition after two weeks.

It serves as the solution for inducing apatite deposition on crosslinked collagen fibers.

To facilitate collagen crosslinking and support apatite mineralization on the collagen matrix.

It suggests the complex has sufficient elasticity and structural integrity for bone substitute applications.

It was implanted in muscle tissue and observed for resorption and cytotoxic effects.

Related Experiment Videos

Main Methods:

The researchers used reconstituted type I collagen and sheet collagen as base materials. They crosslinked the collagen in the presence of alkaline phosphatase and egg-yolk phosvitin. The crosslinked collagen was immersed in calcium beta-glycerophosphate solutions at pH 9.0 and 37 degrees Celsius. The solutions were renewed daily for periods of two and four weeks. This process allowed apatite to deposit on the collagen fibers over time. The weight of the apatite deposits was measured relative to the collagen matrix. The researchers tested the complex's elasticity and resistance to deformation using manual and mechanical methods. They also immersed the complex in saline and animal blood to evaluate its stability and disintegration resistance.

Main Results:

Apatite deposition increased the complex's weight by approximately twice that of the crosslinked collagen. After two weeks of immersion, the apatite layer remained intact and elastic under physical stress. No visible apatite detachment occurred when the complex was manipulated with fingers or forceps. The complex did not disintegrate when exposed to saline or animal blood solutions. When implanted in muscle tissue, the complex resorbed without causing cytotoxic effects. The apatite-collagen structure retained its form during mechanical deformation tests. The results suggest the complex has sufficient structural integrity for bone substitute applications. The findings indicate that the complex could be suitable for periodontal and alveolar ridge repair procedures.

Conclusions:

The apatite-collagen complex developed in this study shows promise as a bone substitute material. The complex maintained elasticity and structural stability under physical stress tests. It resisted disintegration in saline and blood solutions, suggesting durability in biological environments. The complex resorbed safely in muscle tissue without cytotoxic effects. These properties align with the desired characteristics of natural bone substitutes. The study supports the potential use of the complex in periodontal and alveolar bone repair. The findings suggest that the complex could serve as a viable alternative to current materials. The results indicate that the complex may offer improved performance in clinical settings.

The authors suggest it may be useful for periodontal osseous lesion repair and alveolar ridge augmentation.