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Morphological and hemostatic changes in rats with abdominal arterial prosthesis
E Chabielska1, V Kolpakov, M C D'Adamo
1Laboratory of Thrombosis Pharmacology, Istituto di Ricerche Farmacologiche Mario Negri, Consorzio Mario Negri Sud, Santa Maria Imbaro, Italy.
Thrombosis Research
|April 1, 1996
Summary
Arterial thrombosis in rat aortic prostheses involves platelet aggregation and fibrin formation. Systemic hemostatic factors like prostacyclin, fibrinogen, and anticoagulants increase during thrombus development.
Area of Science:
- Cardiovascular Research
- Hematology
- Biomaterials Science
Background:
- Arterial thrombosis is a critical complication of vascular prostheses.
- Understanding the dynamic changes in hemostatic factors during prosthesis-induced thrombosis is crucial for developing effective treatments.
Purpose of the Study:
- To evaluate temporal changes in hemostatic factors during arterial thrombosis induced by a rat aortic prosthesis.
- To investigate local thrombus growth and systemic prostacyclin and tissue plasminogen activator (t-PA) production in parallel.
Main Methods:
- Induction of arterial thrombosis using a loop-shaped aortic prosthesis in rats.
- Monitoring local thrombus formation (platelet aggregation, fibrin network).
- Assessing systemic hemostatic factors including prostacyclin (PGI2), t-PA, fibrinogen, antithrombin III (ATIII), and heparin cofactor II (HCII).
Main Results:
- Platelet activation and spread on the prosthesis within minutes, with aggregate formation supported by fibrin within 24 hours.
- No significant platelet activation observed in peripheral blood.
- Sustained increase in prostacyclin (PGI2) formation and progressive rise in plasma fibrinolytic activity during thrombus growth.
- Steady increase in fibrinogen, antithrombin III (ATIII), and heparin cofactor II (HCII) levels in animals with prostheses.
Conclusions:
- Dynamic phases of thrombus formation in aortic prostheses alter vascular function.
- Systemic levels of key hemostatic factors are significantly affected during ongoing thrombosis.