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[The clonal origin of multiple myeloma]
Zhonghua Nei Ke Za Zhi
|October 1, 1995
Summary
Peripheral blood of multiple myeloma patients contains malignant progenitor cells at various differentiation stages. These cells may home to bone marrow, supporting autologous stem cell transplantation strategies.
Area of Science:
- Hematology
- Immunology
- Oncology
Background:
- Multiple myeloma (MM) is a hematologic malignancy characterized by plasma cell proliferation.
- Understanding the clonal origin and malignant pathway of MM is crucial for effective treatment.
Purpose of the Study:
- To investigate the differentiation stage of malignant progenitor cells in the peripheral blood (PB) of MM patients.
- To explore the clonal origin and malignant pathway of MM.
Main Methods:
- Immunophenotyping of mononuclear cells (MC) in PB and bone marrow (BM) from 17 MM patients using APAAP method and monoclonal antibodies for B-cell markers (CD10, CD19, CD20, CD45RA, CD45RO, CD38).
Main Results:
- No plasma cells were detected in PB samples.
- Peripheral blood mononuclear cells (PBMC) from 10 out of 17 patients expressed CD38, a plasma cell antigen, significantly different from normal controls and identical to BM.
- PBMCs exhibited diverse B-cell marker expression, indicating the presence of precursors at multiple differentiation stages (early B cell to preplasma cell).
- CD45RO expression on PB preplasma cells suggests homing to BM for differentiation into plasma cells.
Conclusions:
- Peripheral blood of MM patients harbors malignant cells at various differentiation stages.
- These findings provide a theoretical basis for purging PB in autologous peripheral blood stem cell transplantation.