[Multiple analysis for the c-Ha-ras activation and DNA content in human gastric precancerous lesions]

The relationship between ras activation and over expression of p21 protein and DNA content in progression from benign to malignant of gastric mucosa was studied. The point mutation at 12 of c-Ha-ras oncogene was detected with PCR and specific oligonucleotide probe hybridization, the expression of ras p21 and DNA ploidy by flow cytometry was also carried out simultaneously on 66 human gastric tissues with gastric cancer and precancerous lesions, some of the patients were followed up for 42 to 60 months. The results showed that: activation of ras oncogene and p21 overexpression may be involved in the early stages of gastric cancer; DNA aneuploidy may not help for the early diagnosis, but can be useful for the diagnosis of gastric cancer in pathology; gastric cancer with mutational activation at 12 of c-Ha-ras may has unfavourable future. It concluded that c-Ha-ras activation and p21 overexpression may be help to early diagnosis of gastric cancer in clinic.