Related Experiment Videos
Isolation and characterization of the restriction endonuclease PpeI from Phormidium persicinum
S Piechula1, J Piosik, K Bielawski
1Department of Microbiology, University of Gdańsk, Poland.
Molecular Biotechnology
|April 1, 1996
Abstract:
PpeI is a type II restriction endonuclease isolated from cyanobacterial strain Phormidium persicinum. The endonuclease PpeI, an isoschizomer of ApaI, recognizes the hexanucleotide sequence (5'-GGGCC/C-3') and cleaves, after the second C, producing four nucleotide 3'-cohesive ends.