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Updated: Aug 10, 2026

Vibratome Sectioning for Enhanced Preservation of the Cytoarchitecture of the Mammalian Organ of Corti
Published on: June 17, 2011
Three-dimensional reconstruction of the cochlea from two-dimensional images of optical sections
1Center for Bioengineering, University of Washington, Seattle 98195, USA.
Abstract:
This paper describes a methodology for three-dimensional (3D) computer-aided reconstruction of the guinea pig cochlea using orthogonal-plane fluorescence optical sectioning. Specimens are sectioned optically, allowing them to remain intact during observation. Equations to correct the data for specimen translation and rotation are developed and 3D reconstructions of the scala tympani, round window membrane, and cochlear aqueduct are presented. The error associated with the reconstruction is estimated to be < 19 microns.
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