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Detection of enteroviruses from clinical specimens by spin amplification shell vial culture and monoclonal antibody
S L Klespies1, D E Cebula, C L Kelley
1Department of Pathology and Laboratory Medicine, Children's Hospital Medical Center of Akron, Ohio 44308, USA.
Abstract:
Conventional tube cell culture was compared with a 72-h, spin-amplified shell vial indirect immunofluorescence assay for the detection of enterovirus from clinical specimens. The sensitivity for the shell vial assay after resolution of discrepant results were 93 and 100%, respectively. The shell vial assay detected 93% of the positive cultures within 72 h of incubation while conventional tube culture detected only 51% of the positive cultures within the same time interval. The data suggest that a spin-amplified shell vial indirect immunofluorescence assay may be useful for the detection of enterovirus from clinical specimens.