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Detection of hepatitis E virus RNA in stools and serum by reverse transcription-PCR
1Service de Bactériologie-Virologie, Hôpital Antoine Béclère, Paris, France.
Journal of Clinical Microbiology
|June 1, 1996
Abstract:
Stools and sera collected during an experimental hepatitis E virus (HEV) infection in monkeys and collected from humans with acute HEV infections during epidemic and sporadic cases were analyzed by reverse transcription-PCR. Two methods for RNA purification were compared. Proteinase K digestion and phenolchloroform extraction were more efficient than guanidinium isothiocyanate extraction in improving the sensitivity and specificity for the detection of HEV genomes.