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An improved DNA test for bovine leucocyte adhesion deficiency
I Tammen1, H Klippert, A Kuczka
1Institute for Animal Breeding and Genetics, Hanover School of Veterinary Sciences, Germany.
Research in Veterinary Science
|May 1, 1996
Summary
A new DNA test for bovine leucocyte adhesion deficiency (BLAD) simplifies diagnosis and breeding schemes. Continuous screening of bulls is recommended to further reduce the incidence of this genetic disease in cattle.
Area of Science:
- Animal genetics
- Veterinary diagnostics
- Molecular biology
Background:
- Bovine leucocyte adhesion deficiency (BLAD) is a monogenic recessive disease affecting cattle.
- Accurate genetic testing is crucial for managing and eradicating BLAD in breeding populations.
Purpose of the Study:
- To develop and validate a modified DNA test for bovine leucocyte adhesion deficiency (BLAD).
- To assess the utility of the test in cattle breeding programs and clinical diagnosis.
- To monitor the carrier rate of BLAD in a large cattle population over time.
Main Methods:
- Development of a modified DNA test utilizing the polymerase chain reaction (PCR).
- Selection of novel primers to improve the clarity and interpretation of test results.
- Analysis of 2381 cattle samples over a three-year period.
Main Results:
- The modified DNA test provides an easily scorable banding pattern, enhancing diagnostic efficiency.
- The carrier rate among young bulls at artificial insemination (AI) stations decreased from 11.6% in 1993 to 9.9% by mid-1995.
- No increase in carrier rates was observed in clinically suspect animals, suggesting carriers are not immunodeficient.
Conclusions:
- The developed DNA test is effective for BLAD diagnosis and integration into cattle breeding schemes.
- Continued screening of young bulls entering AI programs is advised, especially if parental genetic status is unknown.
- Despite eradication efforts, BLAD-affected calves were still identified, highlighting the need for ongoing vigilance.