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Related Experiment Videos

Lead alters structure and function of mouse flexor muscle

A H al Dhaheri1, F F el-Sabban, M A Fahim

  • 1Hematology Department, Al Ain Hospital, Ministry of Health, Al Ain, United Arab Emirates.

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
|April 1, 1996
PubMed
Summary

Chronic exposure to lead acetate in mice significantly reduces skeletal muscle twitch tension and accelerates fatigue. High doses also cause ultrastructural damage to neuromuscular junctions and muscle mitochondria, impacting muscle function.

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Area of Science:

  • Toxicology
  • Skeletal Muscle Physiology
  • Neurotoxicology

Background:

  • Heavy metal exposure, particularly lead (Pb2+), poses a significant threat to human health.
  • Skeletal muscle function can be adversely affected by environmental toxins.
  • Understanding the specific impacts of lead on muscle physiology is crucial for public health.

Purpose of the Study:

  • To investigate the long-term effects of chronic lead acetate exposure on skeletal muscle contractile properties in mice.
  • To analyze dose-dependent effects of lead on muscle performance and identify potential ultrastructural changes.

Main Methods:

  • Male mice received daily subcutaneous injections of lead acetate (0.1 mg/kg or 1 mg/kg) or saline (control) for 7 days.
  • In situ isometric contractile characteristics of the dorsiflexor muscle were measured.

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  • Ultrastructural analysis of neuromuscular junctions and muscle tissue was performed using electron microscopy.

    • Main Results:

    • Chronic lead exposure significantly reduced muscle twitch tension in a dose-dependent manner.
    • Lead treatments accelerated muscle fatigue equally in both low and high dose groups.
    • High-dose lead exposure induced ultrastructural damage, including mitochondrial disruption and synaptic vesicle reduction.

    Conclusions:

    • Chronic exposure to lead acetate impairs skeletal muscle isometric contraction, reducing twitch tension and increasing fatigue.
    • Lead-induced ultrastructural changes at the neuromuscular junction and within muscle fibers contribute to functional deficits.
    • Even low concentrations of lead can compromise skeletal muscle function, highlighting the need for minimizing environmental exposure.