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Related Experiment Videos

Tenascin: growth and adhesion modulation--extracellular matrix degrading function: an in vitro study

P Shrestha1, S Sumitomo, C H Lee

  • 1Department of Oral and Maxillofacial Surgery, Asahi University School of Dentistry, Tsukuba City, Japan.

European Journal of Cancer. Part B, Oral Oncology
|March 1, 1996
PubMed
Summary

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Tenascin (TN), an extracellular matrix protein, promotes tumor cell growth and detachment. Its enhanced expression in cancer may influence tumor progression, adhesion, invasion, and metastasis.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Cancer Research

Background:

  • Tenascin (TN) is an extracellular matrix protein with restricted adult expression, but upregulated in neoplastic lesions.
  • The precise functions and molecular mechanisms of enhanced TN expression in cancer remain unclear.

Purpose of the Study:

  • To investigate the biological roles of Tenascin (TN) in vitro using various cell lines.
  • To elucidate the effects of TN on cell growth, adhesion, and extracellular matrix degradation in neoplastic cells.

Main Methods:

  • In vitro studies using human carcinoma (SCCKN), adenocarcinoma (SGT-1), and mouse fibroblast cell lines (NIH3T3-3, Cle-H3).
  • Assessing TN secretion in response to transforming growth factor-beta.
  • Analyzing cell adhesion on substrates coated with fibronectin (FN), laminin (LN), and TN.

Related Experiment Videos

  • Investigating the induction of collagenase secretion by tumor cells on FN/TN substrates.

    • Main Results:

    • Transforming growth factor-beta dose-dependently enhanced TN secretion in all cell lines.
    • TN exhibited growth-enhancing activity and inhibited cell-to-cell contact.
    • Cells adhered poorly to TN, with high TN concentrations inhibiting adhesion to FN in cancer cells.
    • Neoplastic cells on FN/TN substrates secreted collagenase, unlike non-neoplastic cells.

    Conclusions:

    • Enhanced Tenascin (TN) expression in neoplastic lesions may promote tumor growth and detachment.
    • TN can interfere with fibronectin's role in cell adhesion, potentially impacting cell morphology.
    • The induction of collagenase by TN in cancer cells suggests a role in extracellular matrix degradation and tumor invasion.