Replication of hepatitis C virus
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Updated: Nov 5, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
H Yoshikura1, M Hijikata, N Nakajima
1Department of Bacteriology, Faculty of Medicine, University of Tokyo, Japan.
Abstract:
The mode of replication of the hepatitis C virus (HCV) remains poorly understood. Attempts to produce a tissue culture model containing replicating HCV have been largely unsuccessful. Recent studies on sera from patients chronically infected with HCV have shown that viral particles may be found in high- or low-density fractions. High-density fractions contain non-infectious virions whilst infectious particles can be derived from low-density material. Using appropriate infectious fractions we have successfully infected a number of human cell lines allowing studies of HCV replication to be initiated.
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