Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Integrin and matrix molecule expression in cultured skin replacements

H O Rennekampff1, J F Hansbrough, V Woods

  • 1Department of Surgery, University of California, San Diego Medical Center 92103, USA.

The Journal of Burn Care & Rehabilitation
|May 1, 1996
PubMed
Summary

Cultured skin grafts show variable adhesion. Researchers analyzed integrin and matrix molecule expression on different dermal substitutes to improve skin grafting success for wound healing.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Does patient load affect clinical outcome of burn patients in specialized centers? An analysis of the German Burn Registry.

Burns : journal of the International Society for Burn Injuries·2022
Same author

Wireless microcurrent stimulation improves blood flow in burn wounds.

Burns : journal of the International Society for Burn Injuries·2021
Same author

Theoretical basis for optimal surgical incision planning to reduce hypertrophic scar formation.

Medical hypotheses·2020
Same author

[Current Perceptions of Lipofilling on the Basis of the New Guideline on "Autologous Fat Grafting"].

Handchirurgie, Mikrochirurgie, plastische Chirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Handchirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Mikrochirurgie der Peripheren Nerven und Gefasse : Organ der V...·2016
Same author

The cost of post-burn scarring.

Annals of burns and fire disasters·2016
Same author

Impact of scapholunate dissociation on human wrist kinematics.

The Journal of hand surgery, European volume·2015

Area of Science:

  • Regenerative Medicine
  • Tissue Engineering
  • Dermatology

Background:

  • Cultured human keratinocytes (HK) sheets are used for extensive wound treatment.
  • Dermal replacements with cultured skin substitutes may enhance healing.
  • Adhesion and success rates of HK grafts are often unsatisfactory.

Purpose of the Study:

  • To evaluate adhesion molecule (integrin) expression in cultured HK grafts.
  • To analyze matrix molecule distribution on various dermal substitutes.
  • To identify factors influencing HK graft adhesion and success.

Main Methods:

  • Immunohistologic analysis of integrin subunits (alpha 5, alpha 6, alpha v) and matrix molecules (fibronectin, laminin-1, laminin-5).
  • HK cultured on plastic, polyurethane membranes (Hydroderm), and composite grafts (Dermagraft, AlloDerm).

Related Experiment Videos

Main Results:

  • HK sheets showed basal alpha 6 integrin expression.
  • HK on Hydroderm expressed alpha 5, alpha 6, and alpha v integrins.
  • Reduced HK adhesion on AlloDerm compared to Dermagraft.
  • Integrin expression varied with culture time and dermal substitute type.

Conclusions:

  • Dermal substitute choice impacts integrin expression and HK adhesion.
  • AlloDerm maintained alpha 5 and alpha v positivity in basal keratinocytes.
  • Further research is needed to optimize cultured skin grafts for better wound healing outcomes.