Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Histological evaluation of skin reconstruction using artificial dermis

R Matsui1, N Okura, K Osaki

  • 1R & D Center, Terumo Corporation, Kanagawa, Japan.

Biomaterials
|May 1, 1996
PubMed
Summary

An artificial dermis significantly increased dermal thickness and promoted cell proliferation in skin grafts on rats. This collagen matrix is gradually replaced by host tissue, aiding wound healing.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Motion correction in exercise first-pass radionuclide ventriculography without an external point source.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine·1995
Same author

Decreased uptake of iodinated branched fatty acid analog indicates metabolic alterations in ischemic myocardium.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine·1995
Same author

Myocardial oxidative metabolism in hyperthyroid patients assessed by PET with carbon-11-acetate.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine·1995
Same author

Progression of atrophy of the corpus callosum with deterioration of cerebral cortical oxygen metabolism after carotid artery occlusion: a follow up study with MRI and PET.

Journal of neurology, neurosurgery, and psychiatry·1995
Same author

In vivo assessment of glucose metabolism in hepatocellular carcinoma with FDG-PET.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine·1995
Same author

Repeating the avidin "chase" markedly improved the biodistribution of radiolabelled biotinylated antibodies and promoted the excretion of additional background radioactivity.

European journal of cancer (Oxford, England : 1990)·1995

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Dermatology

Background:

  • Skin defects require effective treatments to restore dermal integrity.
  • Artificial dermis offers a potential solution for regenerating damaged skin layers.

Purpose of the Study:

  • To evaluate the efficacy of an artificial dermis in enhancing split-thickness skin autograft take and dermal regeneration.
  • To assess the histological and proliferative responses following artificial dermis implantation.

Main Methods:

  • Application of a collagen-based artificial dermis to full-thickness skin defects in rats.
  • Overlaying split-thickness skin autografts onto the artificial dermis after a two-week interval.
  • Histological analysis and bromodeoxyuridine incorporation assays to assess graft take and cell proliferation.

Related Experiment Videos

Main Results:

  • Artificial dermis pretreatment resulted in a significantly thicker dermal layer (1.02 mm) compared to controls (0.46 mm).
  • Histology showed seamless integration of the skin graft with the artificial dermis, mimicking natural dermis.
  • High cell proliferation was observed in graft basal cells six days post-transplantation.
  • The artificial dermis was gradually remodeled, with 50% and 20% remaining at 12 and 20 weeks, respectively, indicating host tissue replacement.

Conclusions:

  • Artificial dermis effectively supports split-thickness skin autografts, enhancing dermal thickness and promoting rapid cell proliferation.
  • The collagen matrix serves as a scaffold that is gradually replaced by host tissue, facilitating dermal regeneration.