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Related Experiment Videos

The short insulin tolerance test: feasibility study using venous sampling

R P Young1, J A Critchley, P J Anderson

  • 1Department of Clinical Pharmacology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong.

Diabetic Medicine : a Journal of the British Diabetic Association
|May 1, 1996
PubMed
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The short insulin tolerance test (ITT) using venous blood is a reproducible method for measuring insulin sensitivity. Bedside glucose monitoring enhances safety by identifying hypoglycemia risks, with the 0.1 U kg-1 dose proving more reliable.

Area of Science:

  • Endocrinology
  • Metabolic Research
  • Clinical Diagnostics

Background:

  • The short insulin tolerance test (ITT) is a standard method for assessing insulin sensitivity.
  • Challenges include arterialized sampling and the risk of hypoglycemia.
  • Optimizing ITT protocols is crucial for accurate and safe metabolic assessment.

Purpose of the Study:

  • To evaluate the safety and reproducibility of the short insulin tolerance test (ITT) using venous blood sampling.
  • To compare the efficacy of two insulin doses (0.1 U kg-1 and 0.05 U kg-1) in healthy subjects.
  • To assess the utility of contemporaneous bedside glucose monitoring in managing ITT safety.

Main Methods:

  • Healthy subjects underwent the short insulin tolerance test (ITT) with venous blood sampling.

Related Experiment Videos

  • Two insulin doses (0.1 U kg-1 and 0.05 U kg-1) were administered.
  • Whole blood glucose concentrations were measured at 1-minute intervals for estimating plasma glucose decline rate.

    • Main Results:

    • Venous sampling demonstrated reproducible glucose uptake measurements following insulin administration.
    • Intrasubject coefficients of variation (CV) were 9.4% for the 0.1 U kg-1 dose and 15.9% for the 0.05 U kg-1 dose.
    • One subject required intervention for hypoglycemia; bedside monitoring aided risk identification.

    Conclusions:

    • Venous sampling is a reliable alternative for the short insulin tolerance test (ITT).
    • Contemporaneous glucose monitoring improves ITT safety by flagging hypoglycemia risk.
    • The 0.1 U kg-1 insulin dose offers superior reproducibility and comparable safety to the 0.05 U kg-1 dose.