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Related Experiment Video

Updated: May 2, 2026

Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5
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Effect of ascorbic acid on hyperoxic rat astrocytes

P Sharma1

  • 1Department of Physiology, University of Western Ontario, London, Canada.

Neuroscience
|May 1, 1996
PubMed
Summary
This summary is machine-generated.

Ascorbic acid prevents cell swelling and maintains membrane transport in astrocytes exposed to high oxygen. This finding highlights vitamin C

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Hyperoxia, or high oxygen levels, can induce oxidative stress and cellular damage.
  • Astrocytes play crucial roles in brain function and are susceptible to environmental changes.

Purpose of the Study:

  • To investigate the protective effects of ascorbic acid against hyperoxia-induced changes in astrocytes.
  • To assess the impact of ascorbic acid on astrocyte cell size and L-ascorbic acid transport under hyperoxic conditions.

Main Methods:

  • Subcultured rat astrocytes were exposed to hyperoxia (42% oxygen) for 48 hours.
  • Ascorbic acid or vehicle was administered during the hyperoxic exposure.
  • Cell size and glial fibrillary acidic protein (GFAP) expression were quantified using computerized imaging.
  • L-[14C]ascorbic acid uptake was measured to assess transport function.

Main Results:

  • Hyperoxia significantly increased astrocyte cell size, an effect mitigated by ascorbic acid.
  • Hyperoxia impaired Na(+)-dependent L-ascorbic acid uptake in vehicle-treated cells.
  • Ascorbic acid supplementation preserved normal L-ascorbic acid transport rates during hyperoxia.

Conclusions:

  • Ascorbic acid demonstrates a protective role against hyperoxia-induced astrocytic cell swelling.
  • Vitamin C supplementation can maintain astrocyte membrane transport function under high oxygen conditions.
  • These findings suggest a potential therapeutic benefit of ascorbic acid in conditions involving hyperoxic stress in the brain.