Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Streptococcus pyogenes infection in mice

L K Husmann1, D L Dillehay, V M Jennings

  • 1Department of Microbiology and Immunology, Emory University, Atlanta, GA 30322, USA.

Microbial Pathogenesis
|April 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predictors of interferon-gamma release assay results and their association with COVID-19 infection outcomes.

IJTLD open·2024
Same author

Correction to: Characterization of host response, resorption, and strength properties, and performance in the presence of bacteria for fully absorbable biomaterials for soft tissue repair.

Hernia : the journal of hernias and abdominal wall surgery·2024
Same author

Optogenetic stimulation of striatal patches modifies habit formation and inhibits dopamine release.

Scientific reports·2021
Same author

What are the immunological and clinical implications of microchimerism?

BJOG : an international journal of obstetrics and gynaecology·2018
Same author

Safety of eliminating postpartum magnesium sulphate: intriguing but not yet proven.

BJOG : an international journal of obstetrics and gynaecology·2018
Same author

Characterization of host response, resorption, and strength properties, and performance in the presence of bacteria for fully absorbable biomaterials for soft tissue repair.

Hernia : the journal of hernias and abdominal wall surgery·2017

This study developed a mouse model for invasive Streptococcus pyogenes infection. Intratracheal inoculation effectively induced pneumonia and bacteremia, establishing a reliable model for studying streptococcal pathogenesis.

Area of Science:

  • Microbiology
  • Pathogenesis
  • Infectious Disease

Background:

  • Streptococcus pyogenes is a significant human pathogen.
  • Invasive infections require robust animal models to study pathogenesis.

Purpose of the Study:

  • To establish and characterize a reproducible mouse model for invasive Streptococcus pyogenes infection.
  • To investigate the dose-response relationship of S. pyogenes inoculation via intranasal and intratracheal routes.

Main Methods:

  • Female C3HeB/FeJ mice were inoculated with Streptococcus pyogenes strain B514-Sm (type M50) via intranasal and intratracheal routes.
  • Dose-response effects and resulting pathology, including pneumonia and bacteremia, were assessed.
  • Histopathological analysis was used to evaluate lung lesions.

Related Experiment Videos

Main Results:

  • Intratracheal inoculation led to dose-dependent bronchopneumonia and signs of illness.
  • A dose of 1.0 x 10(7) colony-forming units (CFUs) caused respiratory illness in 50% of mice within 72 hours.
  • Bacteremia occurred in two-thirds of intratracheally inoculated mice, indicating systemic spread.

Conclusions:

  • Intratracheal inoculation provides a quantitative and reliable method for modeling invasive S. pyogenes infection in mice.
  • This model is suitable for future research on streptococcal virulence factors and invasive disease pathogenesis.