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Related Experiment Videos

Decrease of ciliary beat frequency by platelet activating factor: protective effect of ketotifen

L Kahhak1, A Roche, C Dubray

  • 1INSERM U 200, Clamart, France.

Inflammation Research : Official Journal of the European Histamine Research Society ... [Et Al.]
|May 1, 1996
PubMed
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Platelet-activating factor (PAF) and its metabolite lyso-PAF significantly inhibit ciliary beat frequency (CBF) in tracheal cells. This effect, mediated by PAF receptors, suggests a role in respiratory clearance and highlights ketotifen

Area of Science:

  • Respiratory Physiology
  • Cellular Biology
  • Pharmacology

Background:

  • Ciliary beat frequency (CBF) is crucial for mucociliary transport in the respiratory tract.
  • Platelet-activating factor (PAF) and its derivatives are implicated in inflammatory and physiological processes.

Purpose of the Study:

  • To investigate the effects of PAF and its metabolite lyso-PAF on guinea-pig tracheal ciliary beat frequency.
  • To explore the mechanisms underlying PAF-induced CBF changes, including receptor involvement and the influence of specific drugs.

Main Methods:

  • Incubation of guinea-pig tracheal rings with PAF, lyso-PAF, or lyso-phosphatidylcholine (LPC).
  • Assessment of CBF using microphotooscillography.
  • Examination of the effects of PAF receptor antagonist WEB 2086, ketotifen, and pyribenzamine on PAF-induced CBF changes.

Related Experiment Videos

  • Metabolism studies of radiolabeled PAF and lyso-PAF in tracheal rings.

    • Main Results:

    • PAF (C16, C18, C16/C18) and lyso-PAF significantly inhibited tracheal CBF after 3-6 hours of incubation.
    • LPC did not affect CBF, while PAF and lyso-PAF showed potent inhibition.
    • The PAF receptor antagonist WEB 2086 blocked the inhibitory effects of PAF and lyso-PAF on CBF.
    • Ketotifen, but not pyribenzamine, suppressed the PAF and lyso-PAF-induced decrease in CBF.
    • Tracheal rings partially metabolized both PAF to lyso-PAF and lyso-PAF to PAF.

    Conclusions:

    • PAF and lyso-PAF inhibit tracheal ciliary beat frequency, likely through a specific PAF receptor.
    • The findings suggest a role for PAF in modulating pulmonary clearance mechanisms.
    • Ketotifen's ability to inhibit PAF-induced CBF reduction may contribute to its therapeutic efficacy in allergic conditions.