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Related Experiment Videos

A structurally derived consensus pattern for theta class glutathione transferases

J Rossjohn1, P G Board, M W Parker

  • 1Ian Potter Foundation Protein Crystallography Laboratory, St. Vincent's Institute of Medical Research, Fitzroy, Victoria, Australia.

Protein Engineering
|April 1, 1996
PubMed
Summary
This summary is machine-generated.

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Researchers identified a unique sequence pattern for theta class glutathione transferases (GSTs) using crystal structure analysis. This pattern helps identify new GST superfamily members and reveals stress-related proteins belong to the theta class GST family.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Genomics

Background:

  • Glutathione transferases (GSTs) are crucial enzymes involved in detoxification.
  • Different classes of GSTs exist, each with distinct structural and functional properties.
  • The structural basis for distinguishing theta class GSTs from other classes remained unclear.

Purpose of the Study:

  • To determine the first crystal structure of a theta class glutathione transferase.
  • To identify conserved amino acid residues that characterize the theta class GSTs.
  • To discover novel theta class GST members within sequence databases.

Main Methods:

  • X-ray crystallography to determine the 3D structure of a theta class GST.
  • Amino acid sequence alignment guided by the crystal structure.

Related Experiment Videos

  • Bioinformatic scanning of sequence databases using the identified consensus pattern.

    • Main Results:

    • The first crystal structure of a theta class glutathione transferase was determined.
    • A consensus residue pattern was identified, capable of classifying proteins within the GST superfamily and specifically identifying theta class members.
    • Unique theta class residues are concentrated on a structurally diverse hydrophilic surface region and flanking loops of helix 2.
    • Database searches revealed several stress-related proteins as potential new members of the theta class GST family.

    Conclusions:

    • The determined crystal structure provides a template for understanding theta class GSTs.
    • The identified consensus sequence pattern is a reliable marker for theta class GST identification.
    • This finding expands the known repertoire of theta class GSTs to include stress-related proteins.