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Theoretical permutation gel electrophoretic analysis of a curved DNA fragment located in circular permutation
T M Nair1, K Madhusudan, V Nagaraja
1Chemical Engineering Division, National Chemical Laboratory, Pune, India.
Electrophoresis
|April 1, 1996
Abstract:
Using the theoretical model for DNA curvature, we analyzed a set of fragments with a curved insert located in circular permutation. The theoretical permutation analysis of each of the cyclically located fragments reveals the presence of a shifting molecular bend locus. The delineation of the molecular bend locus associated with the fragments obtained by a second permutation helps in providing an explanation for the differential mobility behavior of the fragments.