Immunochemical identification of prostatic epithelial cells in culture

An indirect immunofluorescence technique for identifying prostatic acid phosphatase was used to evaluate primary prostatic cell cultures and established cell lines of prostatic origin. With the use of this technique, we positively identified acid phosphatase immunochemically of prostatic origin in cell lines EB 33 and MA 160. Other cell lines showing positive immunofluorescence reactions include H 494, H 575, and DU 145.