Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Calbindin D28K forms a Ca(2+)-dissociable complex with mellitin in vitro

V La Bella¹, B K Ho, S H Appel

¹Department of Neurology, Baylor College of Medicine, Houston, Texas, USA.

Biochemistry and Molecular Biology International

|May 1, 1996

Summary

Calbindin D28K (CB) forms a stable complex with mellitin (ME) without calcium, dissociating when calcium is added. This calcium-dependent interaction differs from calmodulin

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Speech-based characterization of dopamine replacement therapy in people with Parkinson's disease.

NPJ Parkinson's disease·2020

Same author

First- and third-generation epidermal growth factor receptor inhibitors mediate distinct phosphoprotein signalling networks: implications for adverse dermatological reactions.

The British journal of dermatology·2020

Same author

Prevalence and characteristics of e-cigarette users among Malaysian current and ex-smokers.

Malaysian family physician : the official journal of the Academy of Family Physicians of Malaysia·2019

Same author

Prevalence of smoking and its associated factors with smoking among elderly smokers in Malaysia: findings from a nationwide population-based study.

Tobacco induced diseases·2016

Same author

Factors influencing the role of primary care providers as gatekeepers in the Malaysian public healthcare system.

Malaysian family physician : the official journal of the Academy of Family Physicians of Malaysia·2015

Same author

Prevalence, awareness, treatment and control of diabetes mellitus among the elderly: The 2011 National Health and Morbidity Survey, Malaysia.

Malaysian family physician : the official journal of the Academy of Family Physicians of Malaysia·2015

Area of Science:

Biochemistry
Molecular Biology
Protein Interactions

Background:

Calbindin D28K (CB) is a calcium-binding protein.
Mellitin (ME) is a polypeptide found in bee venom.
Calcium ions play crucial roles in protein function and regulation.

Purpose of the Study:

To investigate the interaction between Calbindin D28K and mellitin.
To characterize the stoichiometry and calcium dependence of the CB-ME complex.
To compare the binding of CB to ME with that of calmodulin (CM) to ME.

Main Methods:

Non-denaturing polyacrylamide gel electrophoresis (PAGE) to assess complex formation and migration.
Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis to analyze protein components after denaturation.

Related Experiment Videos

Varying calcium concentrations to determine the reversibility and calcium dependence of the complex.

Main Results:

Calbindin D28K forms a stable macromolecular complex with mellitin in the absence of calcium (Ca2+).
The CB-ME complex has a molar ratio of 1:4 (CB:ME) and does not migrate in non-denaturing gels.
The complex is reversibly dissociated by the addition of Ca2+, and both proteins migrate normally after SDS denaturation.
This interaction is distinct from calmodulin's interaction with mellitin, which exhibits different calcium dependence and stoichiometry.

Conclusions:

Calbindin D28K binds to the tetrameric form of mellitin in a calcium-dependent manner.
The binding characteristics suggest a role for CB as a calcium-regulated binding protein.
CB and CM exhibit different modes of interaction with ME under varying calcium conditions.