Mouse oocytes injected with cryopreserved round spermatids can develop into normal offspring
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1Department of Veterinary Science, National Institute of Health, Tokyo, Japan.
Purpose:
This study was performed to determine whether frozen-thawed mouse round spermatids can fertilize oocytes and contribute to normal embryo development.
Methods:
Freshly collected mouse testicular cells were frozen in PBS containing 7.5% glycerol and 7.5% fetal bovine serum. After thawing and removal of the cryoprotectants, round spermatids were selected and injected individually into mature oocytes which had been previously activated with Sr(2+)-containing Ca(2+)-free medium.
Results:
After thawing, 75-85% of testicular cells were alive. About 90% of the oocytes were fertilized by intracytoplasmic injection of frozen-thawed round spermatids; 11% (17/150) of embryos transferred to foster mothers developed into normal offspring.
Conclusions:
Mouse round spermatids can be cryopreserved for production of normal offspring.