Sodium chloride in preparative free-flow cell electrophoresis: recent developments

Three buffer systems for free-flow electrophoresis have been designed, which proved useful for performing cell electrophoresis in the presence of 50 mM NaCl. Each system consists of one central cell suspension buffer with 50 mM NaCl, two margin buffers, and two electrode buffers. With the aid of a bromophenol blue/maxilon blue accumulation test the various buffers were adjusted to ensure a laminar flow and remain unchanged on their way through an electrophoresis chamber.