Related Experiment Videos
Electrophoretic analysis of biliary proteins: application of high resolution two-dimensional polyacrylamide gel
1Department of Medicine II, Klinikum Grosshadern, Ludwig-Maximilians-University Munich, Germany.
Electrophoresis
|March 1, 1996
Abstract:
Native bile samples, dialyzed against double distilled water, fractionated by Sepharose 2B column chromatography, concentrated by ultrafiltration and lyophilized, were solubilized in lysis buffer and studied by two-dimensional electrophoresis (2-DE) with immobilized pH gradients in the first dimension. We counted 70, and 7 spots in 2-DE patterns of bile from patients with cholesterol gallstones and pigment gallstones, respectively.