Bonded peptide stationary phases for the separation of amino acids and peptides using liquid chromatography

Bonded optically active tripeptides have been applied as stationary phases for liquid chromatography. Significant retention variations are shown for some UV absorbing amino acids when compared to a silica gel column using the same mobile phase. The separation of certain isomeric dipeptides has been accomplished using the bonded optically active tripeptide stationary phases. It is shown that these separations are superior to those possible on silica gel, although the efficiencies of the column are low. Phenylthiohydatoin (PTH)-amino acids have also been analyzed using the bonded tripeptide L-Val-L-Ala-L-Ser on silica gel CT and 1% citric acid-water as the mobile phase. No two PTH-amino acids showed the same capacity ratio out of 25 tested and the separation of 15 in one isocratic run is presented. The implications and future for the use of bonded opitcally active peptides as stationary phases for liquid chromatography are discussed.