Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Digital image processing of electron micrographs: the PIC system-III

B L Trus1, E Kocsis, J F Conway

  • 1Computational Bioscience and Engineering Laboratory, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA. trus@helix.nih.gov

Journal of Structural Biology
|January 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Activated STING in a vascular and pulmonary syndrome.

The New England journal of medicine·2014
Same author

Diversity of carbapenemases in clinical isolates of Enterobacteriaceae in Croatia--the results of a multicentre study.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2014
Same author

Structure of the pseudorabies virus capsid: comparison with herpes simplex virus type 1 and differential binding of essential minor proteins.

Journal of molecular biology·2013
Same author

Klebsiella pneumoniae harbouring OXA-48 carbapenemase in a Libyan refugee in Italy.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2013
Same author

Prevalence of plasmid-mediated quinolone resistance determinants in Enterobacteriaceae strains isolated in North-East Italy.

Journal of chemotherapy (Florence, Italy)·2013
Same author

Description and plasmid characterization of qnrD determinants in Proteus mirabilis and Morganella morganii.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2011

The PIC system, a 16-year-old image processing software, has been upgraded to PIC-III for analyzing biological macromolecular structures from electron micrographs. This new version offers enhanced functionality and a user-friendly interface for 3D reconstruction.

Area of Science:

  • Structural biology
  • Biophysics
  • Computational biology

Background:

  • The Protein Information Resource (PIR) is a comprehensive, integrated database of protein sequences and annotations.
  • The PIR system has undergone 16 years of continuous development, focusing on electron micrograph processing for structural analysis of biological macromolecules.
  • Previous versions of PIC were implemented on Digital Equipment Corporation (DEC) systems, with PIC-II described for VAX workstations.

Purpose of the Study:

  • To introduce PIC-III, a significantly upgraded version of the PIC image processing software.
  • To highlight the enhanced functionality and user interface of PIC-III for structural analysis of biological macromolecules.
  • To detail the new features enabling advanced 3D reconstruction of biological structures.

Main Methods:

Related Experiment Videos

  • Development of PIC system software in Fortran and C.
  • Implementation on successive generations of DEC minicomputers and Alpha workstations.
  • Integration of an X-windows menu-driven graphical user interface (GUI).
  • Incorporation of dynamic memory allocation and an open systems approach for interfacing with other software.
  • Development of programs for 3D density map reconstruction of helical filaments and particles.

Main Results:

  • PIC-III runs on Alpha workstations, offering substantial functional improvements over PIC-II.
  • The new GUI is accessible from local or remote X-window terminals.
  • Enhanced capabilities for interfacing with visualization software.
  • Successful reconstruction of 3D density maps for various biological structures.

Conclusions:

  • PIC-III represents a major advancement in image processing software for structural biology.
  • The system's flexibility and enhanced features facilitate detailed analysis of biological macromolecules.
  • The open systems approach and advanced reconstruction capabilities support cutting-edge structural biology research.