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Decrease in high density lipoprotein cholesterol during prolonged storage. CELL Study Group

T Ekbom1, L H Lindholm, J Lanke

  • 1Department of Community Health Sciences Dalby/Lund, Sweden.

Scandinavian Journal of Clinical and Laboratory Investigation
|April 1, 1996
PubMed
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High-density lipoprotein cholesterol (HDL) levels decrease over time when serum or plasma samples are stored frozen at -20°C. This study quantises the rate of HDL cholesterol decline during prolonged sample storage.

Area of Science:

  • Clinical Chemistry
  • Biochemistry
  • Lipid Metabolism

Background:

  • Conflicting data exist regarding the stability of high-density lipoprotein cholesterol (HDL) in frozen serum or plasma.
  • Accurate HDL cholesterol measurements are crucial for cardiovascular risk assessment and lipid-lowering strategy trials.

Purpose of the Study:

  • To statistically evaluate the long-term stability of HDL cholesterol in frozen serum samples.
  • To quantify the rate of HDL cholesterol decrease during prolonged storage at -20°C.

Main Methods:

  • Re-analysis of 85 previously frozen serum samples stored for 26 to 46 months at -20°C.
  • HDL cholesterol was measured using the dextran sulphate 500/Mg2+ method.
  • Linear regression analysis was performed to assess the relationship between storage time and HDL cholesterol levels.

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Main Results:

  • A statistically significant time-related decrease in HDL cholesterol was observed during prolonged storage (p < 0.0005).
  • The regression equation indicated an approximate 1% decrease in HDL cholesterol concentration per month of storage after 6 months.
  • The calculated decrease in HDL cholesterol was 0.05 - 0.008 x (time in months).

Conclusions:

  • Frozen serum samples exhibit a time-dependent decrease in HDL cholesterol concentration when stored at -20°C.
  • This finding has implications for the interpretation of HDL cholesterol results from stored samples in clinical trials and diagnostic settings.
  • Standardized protocols for sample storage and analysis are recommended to ensure accurate lipid measurements.