Abnormalities in type IV collagen immunoreactivity in human laryngeal cancers

Abnormal patterns of expression of the basement membrane type IV collagen are observed in many human cancers. This study examines the immunohistological expression of type IV collagen in the basement membrane in human laryngeal squamous cell carcinomas (SCC) (n = 24). Non-neoplastic vocal cord polyps (n = 4) were used as controls. The formalin-fixed, paraffin-embedded tissues were sectioned and pretreated with protease prior to immunostaining for type IV collagen. There was a statistically significant difference in type IV collagen expression between laryngeal SCC and vocal cord polyps (p = 0.0001; chi 2 test with continuity correction). In laryngeal SCC (n = 24; 100%), type IV collagen distribution was discontinuous and irregular or absent around individual or groups of neoplastic cells. In contrast, all of the cases of vocal cord polyps (n = 4; 100%) displayed a continuous pattern of subepithelial basement membrane type IV collagen. This study has shown that abnormal distribution of type IV collagen occurs in laryngeal squamous cell carcinomas but not in non-neoplastic vocal cord polyps. This may be related to either abnormal synthesis or to the breakdown of the collagen and it may be of use as a potential biological marker in the study of laryngeal carcinogenesis.