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Nucleosome assembly: the CAF and the HAT

P D Kaufman1

  • 1Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. pkauf@mendel.berkeley.edu

Current Opinion in Cell Biology
|June 1, 1996
PubMed
Summary

Chromatin assembly factor-1 (CAF-I) deposits acetylated histones during DNA replication. Mechanistic links between histone acetylation and in vivo/in vitro nucleosome assembly remain unclear.

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • Cell Biology

Background:

  • Chromatin assembly factor-1 (CAF-I) is implicated in de novo nucleosome assembly.
  • Histone acetylation is a key epigenetic modification.
  • The first histone acetyltransferase gene was recently cloned.

Purpose of the Study:

  • To investigate the role of CAF-I in histone deposition during DNA replication.
  • To explore the connection between histone acetylation and nucleosome assembly.

Main Methods:

  • In vitro studies of human CAF-I function.
  • Analysis of CAF-I localization in S-phase cells.

Main Results:

  • Human CAF-I deposits newly synthesized, acetylated histones onto replicated DNA in vitro.
  • CAF-I localizes to DNA replication sites in S-phase cells.

Conclusions:

  • CAF-I plays a significant role in depositing acetylated histones during DNA replication.
  • The precise in vivo and in vitro mechanisms linking histone acetylation to nucleosome assembly require further elucidation.

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