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Related Experiment Videos

Constrained diffusion or immobile fraction on cell surfaces: a new interpretation

T J Feder1, I Brust-Mascher, J P Slattery

  • 1Department of Physics, Cornell University, Ithaca, New York 14853, USA.

Biophysical Journal
|June 1, 1996
PubMed
Summary

The immobile fraction observed in fluorescence photobleaching recovery (FPR) studies of cell membrane proteins may result from anomalous subdiffusion, not immobility. This finding suggests a new model for cell membrane structure and protein lateral mobility.

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Area of Science:

  • Cell Biology
  • Biophysics
  • Membrane Dynamics

Background:

  • Protein lateral mobility in cell membranes is typically measured using fluorescence photobleaching recovery (FPR).
  • Classical FPR interpretation assumes free Brownian diffusion, positing an "immobile fraction" whose origin is unclear.
  • Averaging in FPR masks individual receptor motion details, prompting investigation into alternative interpretations.

Purpose of the Study:

  • To investigate the origin of the "immobile fraction" in FPR data.
  • To reconcile single particle tracking observations of diverse receptor motions with FPR measurements.
  • To propose a revised model for cell membrane structure based on observed protein dynamics.

Main Methods:

  • Utilized single particle tracking (SPT) to monitor individual fluorescently labeled immunoglobulin E (Fc epsilon RI) receptor complexes.

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  • Employed fluorescence photobleaching recovery (FPR) on the same cell system.
  • Developed and applied a new model to analyze FPR data, incorporating anomalous diffusion.

    • Main Results:

    • Single particle tracking confirmed that receptors exhibit both free diffusion and restricted, anomalous diffusion.
    • The "immobile fraction" in FPR data can be explained by particles undergoing anomalous subdiffusion.
    • Anomalous subdiffusion is characterized by mean square displacements growing as a power law with an exponent less than one.

    Conclusions:

    • The "immobile fraction" in FPR is likely not truly immobile but rather exhibits restricted lateral mobility via anomalous subdiffusion.
    • These findings challenge the classical interpretation of FPR data and necessitate a new model for cell membrane structure.
    • Understanding anomalous subdiffusion is crucial for accurately interpreting protein lateral mobility in cell membranes.