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[Detection of anti-Treponema pallidum antibodies with colloidal gold immunoprobes]
J D Burgos1, M Mendoza, J Vinasco
1Laboratorio Biotecnológico, Universidad INCCA de Colombia, Bogotá, S. América.
Background:
The aim of this study was to evaluate the usefulness of colloidal gold probes widely used in histochemistry and electronic microscopy for the serologic diagnosis of syphilis.
Methods:
One hundred eighty-nine sera which had been submitted to the traditional fluorescent trigenemic absorption (FTA-ABS) test and had later been tested with the new colloidal gold probe method (IAT-ABS).
Results:
Out of a total of 103 positive samples by FTA-ABS all were positive when tested by gold immunoprobes (sensitivity 100%). Eighty-six samples which were negative by immunofluorescence were all negative by IAT-ABS (specificity 100%).
Conclusions:
The new method of colloidal gold probes with the current microscopy is useful for confirming serologic diagnosis of syphilis.