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Related Experiment Videos

Biointeractive polymers and tissue engineered blood vessels

H P Greisler1, C Gosselin, D Ren

  • 1Department of Surgery, Loyola University Medical Center, Maywood, IL 60153, USA.

Biomaterials
|February 1, 1996
PubMed
Summary
This summary is machine-generated.

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Researchers can control endothelial cell (EC) and smooth muscle cell (SMC) growth in biomaterials by adjusting the ratio of fibroblast growth factor-1 (FGF-1) to heparin. This method offers precise modulation of cell proliferation for vascular interventions.

Area of Science:

  • Biomaterials Science
  • Vascular Biology
  • Regenerative Medicine

Background:

  • Endothelial cell (EC) and smooth muscle cell (SMC) proliferation is crucial for vascular intervention success.
  • Biomaterials can be impregnated with proteins to modulate cell growth.
  • Previous studies showed FGF-1 and heparin in ePTFE grafts increased EC and SMC proliferation.

Purpose of the Study:

  • To investigate the effects of FGF-1, heparin, and thrombin concentrations on SMC proliferation in vitro.
  • To determine if the FGF-1:heparin ratio can differentially modulate EC and SMC growth.
  • To explore the potential of this system for other bioactive proteins.

Main Methods:

  • In vitro study of SMC DNA synthesis in response to varying concentrations of FGF-1, heparin, and thrombin within fibrin glue (FG).

Related Experiment Videos

  • Assessment of EC proliferation synergism with FGF-1 and heparin.
  • Analysis of dose-dependent effects of growth factors and inhibitors.

    • Main Results:

    • Fibrin glue alone significantly increased SMC DNA synthesis.
    • Heparin inhibited FG-induced SMC proliferation in a dose-dependent manner, with complete blockage at 500 U/mL.
    • FGF-1 and heparin together showed a dose-dependent effect on SMC proliferation, with higher heparin concentrations decreasing growth.
    • A synergistic effect of FGF-1 and heparin on EC proliferation was observed without inhibition at higher heparin levels.
    • Thrombin > 3.2 U/mL stimulated SMC growth, which was blocked by heparin.

    Conclusions:

    • The ratio of FGF-1 to heparin can be altered to modulate the relative proliferative activity of ECs versus SMCs.
    • This protein-impregnation system offers a method for controlling cell growth in biomaterials.
    • The system holds potential for inducing local or systemic effects using various bioactive proteins.