Surface plasmon resonance studies of immunoreactions utilizing disposable diffraction gratings

Area of Science:

• Biophotonics
• Biosensing Technology
• Surface Chemistry

Background:

• Surface plasmon resonance (SPR) is a label-free optical technique widely used for studying biomolecular interactions.
• Prisms are conventionally employed as momentum-coupling devices in SPR biosensors.
• There is a need for cost-effective and disposable sensing components in biosensor development.

Purpose of the Study:

• To investigate the efficiency of perspex holographic diffraction grating replicas as momentum-coupling devices in SPR.
• To compare the performance of grating replicas with traditional prism-based systems.
• To assess the suitability of grating replicas for disposable biosensor heads.

Main Methods:

• Surface plasmon resonance (SPR) measurements were conducted.
• The binding specificity between immuno-gamma globulin (IgG) and anti-IgG was analyzed.
• Holographic diffraction grating replicas were fabricated and tested as coupling devices.

Main Results:

• Holographic diffraction grating replicas demonstrated potential as efficient momentum-coupling devices.
• The study discussed the advantages and disadvantages of grating replicas versus prism systems.
• A sensitive optical technique applicable beyond prism geometries was highlighted.

Conclusions:

• Perspex holographic diffraction grating replicas offer a low-cost, easily fabricated alternative for SPR biosensing.
• These replicas show promise as disposable sensing heads, potentially reducing biosensor costs.
• The findings contribute to the development of novel and accessible biosensing platforms.