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Mapping of transcribed sequences on human chromosome 19
S Volik1, Y Lebedev, L Nikolaev
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Ras, Moscow, Russia.
DNA Sequence : the Journal of DNA Sequencing and Mapping
|January 1, 1995
Summary
This study mapped 30 expressed sequence tags (ESTs) and sequence tagged sites (STSs) on human chromosome 19, identifying gene locations and repeated elements. These mapped sequences offer valuable starting points for systematic genome sequencing efforts.
Area of Science:
- Genomics
- Human Genetics
- Molecular Biology
Background:
- Chromosome 19 contains numerous genes and repetitive elements.
- Efficient mapping of transcribed sequences is crucial for understanding genome organization.
Purpose of the Study:
- To map expressed sequence tags (ESTs) and sequence tagged sites (STSs) on human chromosome 19.
- To identify locations of transcribed units and repetitive elements.
- To provide starting points for systematic genomic sequencing.
Main Methods:
- Utilized a human complementary DNA (hncDNA) library for transcribed sequences.
- Hybridized chromosome 19-specific hncDNA clones to chromosome 19 cosmids.
- Verified hybridization results using Polymerase Chain Reaction (PCR).
Main Results:
- Successfully mapped 30 ESTs/STSs on human chromosome 19.
- Identified over 50 sites belonging to 19 families of related sequences.
- Mapped sequences cover approximately 80% of chromosome 19.
Conclusions:
- The EST mapping approach effectively pinpoints gene locations and repetitive elements.
- Mapped hncDNA sequences are valuable resources for future genomic sequencing projects.
- This study enhances the understanding of human chromosome 19's genetic landscape.