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Related Experiment Videos

Intracytoplasmic sperm injection in the mouse

R Ron-El1, J Liu, Z Nagy

  • 1Department of Obstetrics and Gynecology, Assaf Harofeh Medical Center, Sackler School of Medicine, Tel Aviv University, Israel.

Human Reproduction (Oxford, England)
|November 1, 1995
PubMed
Summary

Intracytoplasmic sperm injection (ICSI) in mice has low success rates. This study found that sperm head escape and tail location post-injection significantly impact oocyte survival and fertilization outcomes in mouse ICSI.

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Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Assisted Reproductive Technologies

Background:

  • Intracytoplasmic sperm injection (ICSI) is a key assisted reproductive technology.
  • Low oocyte survival rates and fertilization failures are common challenges in mouse ICSI models.
  • Understanding failure mechanisms is crucial for improving ICSI protocols.

Purpose of the Study:

  • To investigate the causes of frequent ICSI failure in mouse oocytes.
  • To evaluate the impact of sperm head and tail location on oocyte survival and fertilization.
  • To compare outcomes between non-activated and calcium ionophore-activated oocytes.

Main Methods:

  • ICSI was performed on Metaphase II oocytes from F1 hybrid mice.
  • Spermatozoa were retrieved from the epididymis of F1 hybrid mice.

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  • Oocytes were divided into two groups: non-activated (Group 1) and calcium ionophore-activated (Group 2).
  • Microinjection pipettes with specific outer (10 microns) and inner (8 microns) diameters were used.
  • Oocytes were evaluated at 6, 20, 48, and 72 hours post-injection.

    • Main Results:

    • In Group 1 (non-activated), 47% of oocytes were damaged or degenerated, with 53% survival but no fertilization.
    • In Group 2 (calcium ionophore-activated), 22% of oocytes were damaged, with 78% survival and 4.2% fertilization rate.
    • Sperm head escape into the perivitelline space occurred in Group 1.
    • Sperm tail location varied: cytoplasm (27-31%), perivitelline space (45%), or protruding zona pellucida (23-28%).
    • Higher degeneration rates were observed when sperm tails remained in the cytoplasm (78% in Group 1, 36% in Group 2).

    Conclusions:

    • Sperm head escape and improper tail localization are significant contributors to ICSI failure in mice.
    • Oocyte activation status influences survival and fertilization rates post-ICSI.
    • Further optimization of ICSI techniques is needed to improve success rates in mouse models.