Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CD4+ cells in human ejaculates

T Gil1, J A Castilla, M L Hortas

  • 1Servicio Analisis Clinicos, Hospital Virgen de las Nieves, Granada, Spain.

Human Reproduction (Oxford, England)
|November 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ontogeny and organ-specific steroidal glycoside diversity is associated with differential expression of steroidal glycoside pathway genes in two Solanum dulcamara leaf chemotypes.

Plant biology (Stuttgart, Germany)·2024
Same author

OVOL1 immunohistochemical expression is a useful tool to diagnose invasion in ocular surface squamous neoplasms.

Archivos de la Sociedad Espanola de Oftalmologia·2022
Same author

Transition from unclassified Ktedonobacterales to Actinobacteria during amorphous silica precipitation in a quartzite cave environment.

Scientific reports·2021
Same author

Autologous stem cell transplantation for untreated transformed indolent B-cell lymphoma in first remission: an international, multi-centre propensity-score-matched study.

British journal of haematology·2020
Same author

Targeting nucleolin for better survival in diffuse large B-cell lymphoma.

Leukemia·2017
Same author

Evaluation of learned helplessness, self-efficacy and disease activity, functional capacity and pain in Argentinian patients with rheumatoid arthritis.

Scandinavian journal of rheumatology·2016
Same journal

Characterizing endometriosis and adenomyosis symptom clusters and their impact on quality of life in the All of Us Research Program.

Human reproduction (Oxford, England)·2026
Same journal

A new regulatory framework: impact of the SoHO Regulation for medically assisted reproduction.

Human reproduction (Oxford, England)·2026
Same journal

Subfertility in relation to gestational weight gain and gestational diabetes: the Norwegian Mother, Father and Child Cohort Study.

Human reproduction (Oxford, England)·2026
Same journal

Pathogenic variants in ACTRT3 lead to male infertility characterized by fertilization defects.

Human reproduction (Oxford, England)·2026
Same journal

A large retrospective study on 1PN embryo transfer supports the need for updated harmonized best practice guidelines.

Human reproduction (Oxford, England)·2026
Same journal

Explainability of artificial intelligence (AI) tools in assisted reproductive technologies (ARTs): promise, clinical reality, and conceptual limits.

Human reproduction (Oxford, England)·2026
See all related articles

Human spermatozoa do not express the CD4 antigen, a key receptor for HIV. Researchers found CD4 antigen only on leukocytes, specifically T lymphocytes and monocytes, within semen samples.

Area of Science:

  • Immunology
  • Reproductive Biology

Background:

  • The CD4 antigen is a cell surface receptor crucial for human immunodeficiency virus (HIV) entry.
  • Understanding CD4 antigen expression in semen is vital for reproductive health and HIV transmission research.

Purpose of the Study:

  • To investigate the presence and location of CD4 antigen expression in human ejaculate.
  • To determine if human spermatozoa express the CD4 antigen.

Main Methods:

  • Flow cytometry was used to analyze CD4 antigen expression on spermatozoa and round cells in semen.
  • Peroxidase tests identified leukocytes in semen samples.
  • Swim-up technique was employed for spermatozoa analysis.

Main Results:

  • CD4 antigen was not detected on the surface of human spermatozoa or CD45- ejaculate cells (epithelial, germinal).

Related Experiment Videos

  • CD4 antigen was present on CD45+ leukocyte cells within the ejaculate.
  • Further characterization revealed CD4+ cells in semen comprise T lymphocytes (helper/inducer) and monocytes.

    • Conclusions:

    • Human spermatozoa do not express the CD4 antigen, thus are not a direct target for HIV via this receptor.
    • Semen contains CD4+ T lymphocytes and CD4+ monocytes, indicating immune cell presence in ejaculate.