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A trinucleotide deletion together with a base duplication event at codon 439 in the human tyrosinase gene identifies
L H Breimer1, A F Winder, P Panayiotidis
1Department of Chemical Pathology and Human Metabolism, Royal Free Hospital School of Medicine, London, UK.
Abstract:
Molecular analysis of the human tyrosinase gene in two patients suffering from a temperature-sensitive form of albinism has identified a thymine triplet deletion at codon 439 which is accompanied by a duplication of the immediately preceding cytosine residue. This results in a two base pair frame shift leading to premature termination at codon 448, giving a truncated protein. Its relationship to other mutations in tyrosinase and the possible cause are discussed. The temperature-sensitive phenotype is due to the guanine to adenine mutation at codon 422, known to generate a temperature-sensitive enzyme. The CTTT at F439 in tyrosinase is also present at F508 in CFTR, the main mutation causing cystic fibrosis.
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