Determination of solasodine in fruits of Solanum species

A potentiometric nonaqueous titration procedure was developed for the quantitative determination of solasodine in fruits of the Solanum species. The steroid glycoalkaloids were extracted from freshly harvested fruits with 2% acetic acid and methanol. After hydrolysis, the common aglycone solasodine was extracted with benzene. An aliquot was mixed with an equal volume of acetone and titrated potentiometrically with 0.005 N perchloric acid in dioxane, using glass and silver electrodes for the determination.