GLC determination of free triclocarban in blood

A method is presented for the quantitative determination of free triclocarban in rat or human blood. The procedure involves the extraction from blood with acetone, a TLC cleanup, derivatization with N,O-bis (trimethylsily) acetonide, and GLC using an electron-capture detector. GLC-mass spectral analysis confirmed that the structure of the derivative was a bis (trimethylsilyl) molecule with one group on a nitrogen and the second group attached to the enol tautomer. The method is sensitive to 25 ng (12.5 microgram/liter of blood). Recoveries of added triclocarban in the 12.5--50-microgram/liter range weree between 80 and 90%.