Determination of triprolidine in human plasma by quantitative TLC

A chromatographic thin-layer fluorescence procedure, with a sensitivity limit of 0.8 ng/ml, is described for the quantitative analysis of triprolidine in human and rat plasma. Following the intravenous administration of 1 mg/kg of triprolidine to rats, the drug distributed rapidly into tissues and was eliminated from plasma with a half-life of 53 min. The method was used to determine the plasma triprolidine levels in 16 normal human volunteers following oral administration of 3.75 mg of triprolidine hydrochloride in 15 ml of a syrup. The drug obtained a mean peak plasma level of 8.2 ng/ml in 2 hr and was eliminated from the plasma with a half-life of 5 hr. Considerable individual variation was observed in the area under the plasma triprolidine level-time curve; values ranged from 19 to 163 ng hr/ml with a mean value of 75 ng hr/ml.