Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Stem cell cryopreservation

J Gorlin1

  • 1Division of Hematology Oncology, Children's Hospital, Boston, Massachusetts, USA.

The Journal of Infusional Chemotherapy
|January 1, 1996
PubMed
Summary
This summary is machine-generated.

High-dose chemotherapy necessitates improved cryobiology services for progenitor cells. This review explores the science behind freezing and thawing stem cells, addressing new regulatory standards.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prothrombin complex concentrate for emergent reversal of warfarin: an international survey of hospital protocols.

Vox sanguinis·2017
Same author

International Forum regarding practices related to donor haemoglobin and iron.

Vox sanguinis·2016
Same author

Prevalence of blood donor iron deficiency and feasibility ferritin-based iron replacement: a blood collection agency-based study.

Vox sanguinis·2016
Same author

Pilot study of oxygen transport rate of banked red blood cells.

Vox sanguinis·2009
Same author

Purification of salmon clotting factors and their use as tissue sealants.

Thrombosis research·2001
Same author

Intensive blood and plasma exchange for treatment of coagulopathy in meningococcemia.

Journal of clinical apheresis·1995
Same journal

Neoadjuvant chemotherapy with continuous infusion of cisplatin and 5-fluorouracil, with or without leucovorin, for locally advanced nasopharyngeal carcinoma.

The Journal of infusional chemotherapy·1996
Same journal

Continuous infusion 5-fluorouracil as first-line therapy for metastatic breast cancer.

The Journal of infusional chemotherapy·1996
Same journal

The dead end of 5-fluorouracil double modulation and promise of continuous infusion schedules in the treatment of metastatic colorectal cancer.

The Journal of infusional chemotherapy·1996
Same journal

The French experience with infusional 5-FU in gastric and pancreatic cancer.

The Journal of infusional chemotherapy·1996
Same journal

Drug stability and compatibility in oncology care.

The Journal of infusional chemotherapy·1996
Same journal

Options for venous access in ambulatory care: issues in selection and management.

The Journal of infusional chemotherapy·1996
See all related articles

Area of Science:

  • Cryobiology
  • Hematopoietic Stem Cell Transplantation
  • Cellular Cryopreservation

Background:

  • Increased use of high-dose chemotherapy with peripheral blood progenitor cell rescue drives demand for cryopreservation services.
  • This surge necessitates a reevaluation of existing cryobiological practices and the exploration of alternative methods.
  • Hematopoietic stem cell processing faces evolving regulatory scrutiny from agencies like the FDA.

Purpose of the Study:

  • To review the scientific principles underlying current cryobiological practices.
  • To elucidate the mechanisms of cryoprotectant action.
  • To discuss alternative methodologies for freezing and thawing progenitor cells.

Main Methods:

  • Literature review of cryobiology principles and practices.

Related Experiment Videos

  • Analysis of cryoprotectant function.
  • Examination of emerging freezing and thawing techniques.
  • Assessment of the regulatory landscape for stem cell processing.

    • Main Results:

    • Cryoprotectants function by reducing intracellular ice formation and solution effects.
    • Alternative methods aim to improve cell viability and reduce cryodamage.
    • Regulatory bodies are increasing oversight of hematopoietic stem cell processing.

    Conclusions:

    • Optimizing cryopreservation techniques is crucial for effective stem cell transplantation.
    • Understanding cryoprotectant mechanisms aids in developing better protocols.
    • Adherence to emerging regulatory standards is essential for stem cell processing facilities.