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Murine monoclonal antibody recognizing human alpha(1,3/1,4)fucosyltransferase
H Kimura1, T Kudo, S Nishihara
1Institute of Life Science, Soka University, Tokyo, Japan.
Glycoconjugate Journal
|December 1, 1995
Summary
A new antibody, FTA1-16, specifically targets alpha(1,3/1,4)fucosyltransferase, an enzyme linked to cancer. This antibody shows promise for detecting cancer in digestive organs and colon tissues.
Area of Science:
- Biochemistry
- Immunology
- Oncology
Background:
- Alpha(1,3/1,4)fucosyltransferase enzymes play a role in glycosylation.
- Aberrant expression of fucosylated antigens is associated with cancer progression.
- Specific antibodies are needed to study these enzymes in disease contexts.
Purpose of the Study:
- To develop and characterize a novel monoclonal antibody, FTA1-16, for specific detection of human alpha(1,3/1,4)fucosyltransferase.
- To investigate the expression patterns of alpha(1,3/1,4)fucosyltransferase in various human tumor cell lines and tissues.
- To identify the specific epitope recognized by the FTA1-16 antibody.
Main Methods:
- Production and characterization of mouse monoclonal antibody FTA1-16.
- Immunofluorescence staining of Golgi apparatus and Western blotting using transfected cell lines.
- Western blotting and immunohistochemical staining of human tumor cell lines and colon cancer tissues.
- Epitope mapping using truncated recombinant peptides expressed in E. coli.
Main Results:
- FTA1-16 demonstrated high specificity for human alpha(1,3/1,4)fucosyltransferase, with no cross-reactivity to other alpha(1,3)fucosyltransferase family members.
- Expression of alpha(1,3/1,4)fucosyltransferase correlated with sialyl Lewis a antigen in epithelial cancer cell lines from digestive organs.
- Immunohistochemical analysis revealed enhanced expression of alpha(1,3/1,4)fucosyltransferase in colon cancer cells compared to normal cells.
- The minimal antigenic epitope recognized by FTA1-16 was mapped to amino acid positions 132-153 of the enzyme.
Conclusions:
- The monoclonal antibody FTA1-16 is a specific and valuable tool for detecting human alpha(1,3/1,4)fucosyltransferase.
- Elevated expression of alpha(1,3/1,4)fucosyltransferase is observed in certain cancers, particularly in digestive organs and colon.
- FTA1-16 holds potential for cancer diagnostics and research, especially in identifying fucosylated biomarkers.