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Cytocidal infection of hog cholera virus in porcine bone marrow stroma cell cultures
M Shimizu1, S Yamada, T Nishimori
1National Institute of Animal Health, Ibaraki, Japan.
Abstract:
Porcine bone marrow stroma cell (BMSC) cultures producing cells of granulocyte-lineage were established. Hog cholera (HC) virus ALD and Alfort strains replicated in the porcine BMSC cultures showing distinct cytopathic effect (CPE). The differentiation of granulocyte-lineage cells in the cultures ceased after infection with HC virus. Polyclonal antibody against the ALD strain inhibited completely the development of CPE of the both ALD and Alfort strains. Monoclonal antibodies (mAbs) specific to the ALD strain inhibited CPE of the ALD strain, while CPE of the Alfort strain was not affected by those mAbs, suggesting that CPE induced in the BMSC cultures is due to HC virus.