Proteins at atomic resolution
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Updated: Aug 17, 2026

Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition
Published on: December 22, 2011
Z Dauter1, V S Lamzin, K S Wilson
1European Molecular Biology Laboratory, Hamburg, Germany.
Abstract:
Experimental advances in data collection, including bright sources, cryogenic cooling and two-dimensional detectors, have made it tractable to record data to beyond 1.2 A for several proteins, yielding high-accuracy models and fine details of structure. For small metalloproteins, atomic-resolution data have enabled ab initio solution of the phase problem.
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